DetoxFungiV1, Main, Exploration, bibRecord, 000820

Metagenomic sequencing provides insights into microbial detoxification in the guts of small mammalian herbivores (Neotoma spp.).

Identifieur interne : 000820 ( Main/Exploration ); précédent : 000819; suivant : 000821

Metagenomic sequencing provides insights into microbial detoxification in the guts of small mammalian herbivores (Neotoma spp.).

Auteurs : Kevin D. Kohl [États-Unis] ; Kelly F. Oakeson [États-Unis] ; Teri J. Orr [États-Unis] ; Aaron W. Miller [États-Unis] ; Jennifer Sorensen Forbey [États-Unis] ; Caleb D. Phillips [États-Unis] ; Colin Dale [États-Unis] ; Robert B. Weiss [États-Unis] ; M Denise Dearing [États-Unis]

Source :

FEMS microbiology ecology [ 1574-6941 ] ; 2018.

RBID : pubmed:30202961

Descripteurs français

KwdFr :
- Animaux (MeSH), Cactaceae (composition chimique), Caecum (métabolisme), Herbivorie (physiologie), Inactivation métabolique (génétique), Inactivation métabolique (physiologie), Juniperus (composition chimique), Microbiote (génétique), Métagénomique (MeSH), Oxalates (analyse), Phénols (analyse), Sigmodontinae (classification), Sigmodontinae (microbiologie), Sigmodontinae (métabolisme), Terpènes (analyse).
MESH :
- analyse : Oxalates, Phénols, Terpènes.
- composition chimique : Cactaceae, Juniperus, Sigmodontinae.
- génétique : Inactivation métabolique, Microbiote.
- microbiologie : Sigmodontinae.
- métabolisme : Caecum, Sigmodontinae.
- physiologie : Herbivorie, Inactivation métabolique.
- Animaux, Métagénomique.

English descriptors

KwdEn :
- Animals (MeSH), Cactaceae (chemistry), Cecum (metabolism), Herbivory (physiology), Inactivation, Metabolic (genetics), Inactivation, Metabolic (physiology), Juniperus (chemistry), Metagenomics (MeSH), Microbiota (genetics), Oxalates (analysis), Phenols (analysis), Sigmodontinae (classification), Sigmodontinae (metabolism), Sigmodontinae (microbiology), Terpenes (analysis).
MESH :
- chemical , analysis : Oxalates, Phenols, Terpenes.
- chemistry : Cactaceae, Juniperus.
- classification : Sigmodontinae.
- genetics : Inactivation, Metabolic, Microbiota.
- metabolism : Cecum, Sigmodontinae.
- microbiology : Sigmodontinae.
- physiology : Herbivory, Inactivation, Metabolic.
- Animals, Metagenomics.

Abstract

Microbial detoxification of plant toxins influences the use of plants as food sources by herbivores. Stephen's woodrats (Neotoma stephensi) specialize on juniper, which is defended by oxalate, phenolics and monoterpenes, while closely related N. albigula specialize on cactus, which only contains oxalate. Woodrats maintain two gut chambers harboring dense microbial communities: a foregut chamber proximal to the major site of toxin absorption, and a cecal chamber in their hindgut. We performed several experiments to investigate the location and nature of microbial detoxification in the woodrat gut. First, we measured toxin concentrations across gut chambers of N. stephensi. Compared to food material, oxalate concentrations were immediately lower in the foregut, while concentrations of terpenes remained high in the foregut, and were lowest in the cecal chamber. We conducted metagenomic sequencing of the foregut chambers of both woodrat species and cecal chambers of N. stephensi to compare microbial functions. We found that most genes associated with detoxification were more abundant in the cecal chambers of N. stephensi. However, some genes associated with degradation of oxalate and phenolic compounds were more abundant in the foregut chambers. Thus, microbial detoxification may take place in various chambers depending on the class of chemical compound.

DOI: 10.1093/femsec/fiy184
PubMed: 30202961
PubMed Central: PMC6927881

Affiliations:

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term>
<term>Cactaceae (chemistry)</term>
<term>Cecum (metabolism)</term>
<term>Herbivory (physiology)</term>
<term>Inactivation, Metabolic (genetics)</term>
<term>Inactivation, Metabolic (physiology)</term>
<term>Juniperus (chemistry)</term>
<term>Metagenomics (MeSH)</term>
<term>Microbiota (genetics)</term>
<term>Oxalates (analysis)</term>
<term>Phenols (analysis)</term>
<term>Sigmodontinae (classification)</term>
<term>Sigmodontinae (metabolism)</term>
<term>Sigmodontinae (microbiology)</term>
<term>Terpenes (analysis)</term>
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<term>Cactaceae (composition chimique)</term>
<term>Caecum (métabolisme)</term>
<term>Herbivorie (physiologie)</term>
<term>Inactivation métabolique (génétique)</term>
<term>Inactivation métabolique (physiologie)</term>
<term>Juniperus (composition chimique)</term>
<term>Microbiote (génétique)</term>
<term>Métagénomique (MeSH)</term>
<term>Oxalates (analyse)</term>
<term>Phénols (analyse)</term>
<term>Sigmodontinae (classification)</term>
<term>Sigmodontinae (microbiologie)</term>
<term>Sigmodontinae (métabolisme)</term>
<term>Terpènes (analyse)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Oxalates</term>
<term>Phenols</term>
<term>Terpenes</term>
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<term>Phénols</term>
<term>Terpènes</term>
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<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cactaceae</term>
<term>Juniperus</term>
</keywords>
<keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Sigmodontinae</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Cactaceae</term>
<term>Juniperus</term>
<term>Sigmodontinae</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Inactivation, Metabolic</term>
<term>Microbiota</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Inactivation métabolique</term>
<term>Microbiote</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cecum</term>
<term>Sigmodontinae</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Sigmodontinae</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Sigmodontinae</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Caecum</term>
<term>Sigmodontinae</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Herbivorie</term>
<term>Inactivation métabolique</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Herbivory</term>
<term>Inactivation, Metabolic</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Metagenomics</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Métagénomique</term>
</keywords>
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<front><div type="abstract" xml:lang="en">Microbial detoxification of plant toxins influences the use of plants as food sources by herbivores. Stephen's woodrats (Neotoma stephensi) specialize on juniper, which is defended by oxalate, phenolics and monoterpenes, while closely related N. albigula specialize on cactus, which only contains oxalate. Woodrats maintain two gut chambers harboring dense microbial communities: a foregut chamber proximal to the major site of toxin absorption, and a cecal chamber in their hindgut. We performed several experiments to investigate the location and nature of microbial detoxification in the woodrat gut. First, we measured toxin concentrations across gut chambers of N. stephensi. Compared to food material, oxalate concentrations were immediately lower in the foregut, while concentrations of terpenes remained high in the foregut, and were lowest in the cecal chamber. We conducted metagenomic sequencing of the foregut chambers of both woodrat species and cecal chambers of N. stephensi to compare microbial functions. We found that most genes associated with detoxification were more abundant in the cecal chambers of N. stephensi. However, some genes associated with degradation of oxalate and phenolic compounds were more abundant in the foregut chambers. Thus, microbial detoxification may take place in various chambers depending on the class of chemical compound.</div>
</front>
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<Abstract><AbstractText>Microbial detoxification of plant toxins influences the use of plants as food sources by herbivores. Stephen's woodrats (Neotoma stephensi) specialize on juniper, which is defended by oxalate, phenolics and monoterpenes, while closely related N. albigula specialize on cactus, which only contains oxalate. Woodrats maintain two gut chambers harboring dense microbial communities: a foregut chamber proximal to the major site of toxin absorption, and a cecal chamber in their hindgut. We performed several experiments to investigate the location and nature of microbial detoxification in the woodrat gut. First, we measured toxin concentrations across gut chambers of N. stephensi. Compared to food material, oxalate concentrations were immediately lower in the foregut, while concentrations of terpenes remained high in the foregut, and were lowest in the cecal chamber. We conducted metagenomic sequencing of the foregut chambers of both woodrat species and cecal chambers of N. stephensi to compare microbial functions. We found that most genes associated with detoxification were more abundant in the cecal chambers of N. stephensi. However, some genes associated with degradation of oxalate and phenolic compounds were more abundant in the foregut chambers. Thus, microbial detoxification may take place in various chambers depending on the class of chemical compound.</AbstractText>
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<AffiliationInfo><Affiliation>Department of Biology, University of Utah, 257 South 1400 East, Salt Lake City, UT, 84112, USA.</Affiliation>
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<Initials>TJ</Initials>
<AffiliationInfo><Affiliation>Department of Biology, University of Utah, 257 South 1400 East, Salt Lake City, UT, 84112, USA.</Affiliation>
</AffiliationInfo>
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	Serveur d'exploration sur la détoxication des champignons
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Serveur d'exploration sur la détoxication des champignons

Metagenomic sequencing provides insights into microbial detoxification in the guts of small mammalian herbivores (Neotoma spp.).

Metagenomic sequencing provides insights into microbial detoxification in the guts of small mammalian herbivores (Neotoma spp.).

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